- The Ash-throated Flycatcher frequently uses human-made structures for nesting. It readily uses nest boxes, as well as pipes, fence posts, ledges under eaves or porches, and even in clothes hanging on a clothesline.
- Unlike most members of its genus, the Ash-throated Flycatcher only occasionally uses snakeskin in its nest. Only 5% of nests examined contained reptile skin, but 98% had mammal hair (particularly rabbit fur).
- Incubation is done by the female only; the male will bring food and supplementary nest material to the incubating female.